The number, density, volume and morphology of neurons and neuroglia correlate with changes with brain function. The cellular composition of human nervous tissue comprises of three basic cell types: neurons, neuroglia and endothelial cells. Keywords: fundus striati, cell profiler, image analysis IntroductionĬonstant changing patterns of activity of billions of neurons have been extensively studied by thousands of scientists and neurologists as a matter of scientific rigor and most importantly for updating disease prevention and control measures.
#Cellprofiler analyzing lines software#
Cell profiler is cost effective software which is beneficial to identify and measure neurons of fundus striati. Results of the present study can be extrapolated to correlate with pathological conditions associated with emotional and behavioral disorders involving fundus striati. Mean Intensity units of neurons of fundus striati as measured by intensity module of Cell profiler was 17.916 pixel units. Using specific modules, neurons and branching points of their neurites in images of fundus striati were measured in pixel units. Neuronal nuclei were identified as primary objects and cytoplasm was identified by the software as secondary objects. Freely downloadable Cell Profiler software was installed, specific pipeline for neuronal assay was drawn, and modules were used to analyze images. A qualitative cross sectional study was done using eighty one images of histological sections of hematoxylin and eosin stained 8µ thick tissue sections of fundus striati. The present study was undertaken to study and analyze images of fundus striati using cell profiler as an automated image analysis technique. A versatile technique to measure neurons of fundus striati is the use of Cell profiler software. One such part of human brain which has been explored in recent years is fundus striati, a part of basal ganglia bridging caudate nucleus and putamen. Analysis of volumetric and morphological neuronal data has been of keen interest to neurologists and neuroscientists because of its implications in pathological conditions such as schizophrenia, autism, obsessive compulsive disorder etc.
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